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Genomic Science Program

2007 Awardee

Development of Genomic Tools for the Improvement of Prairie Cordgrass( Spartina pectinata ), a Highly Productive Bioenergy Feedstock Crop

INVESTIGATORS: Jose L. Gonzalez, Arvid Boe, XingYou Gu,Vance Owens

INSTITUTION: South Dakota State University

NON-TECHNICAL SUMMARY: Cellulosic biomass has been identified as an attractiverenewable source because of its domestic abundance. Prairie cordgrass(PCG) ( Spartina pectinata ) is a native perennial grass with biomassyields up to 22 metric tons/ha. This species has received little attentionfrom the breeding side, far less from the genomics and molecular biologyperspective.

OBJECTIVES: (1) Development of molecular markers in prairie cordgrass,with especial attention to PCR based marker systems such as SimpleSequence Repeats (SSRs) for use in Marker Assisted Breeding. (2) Developmentof a medium density framework linkage map in prairie cordgrass.

APPROACH: Obj 1. We propose to develop SSR markers for this species bydeveloping enriched libraries with specific sequence repeats, reducingthe sequencing required for marker development. Obj 2. We propose toconstruct the first linkage map available for this very productivespecies. For this, we will use reciprocal crosses between to individuals.The populations will maintain by clonal propagation. The PCR markersand the linkage map will facilitate the development of a breeding programthat will release high-biomass-yielding cultivar of PCG. The linkagemap will also constitute the base for other genomic tools.

Name: Jose L. Gonzalez
Phone: 605-688-6907
Fax: 605-688-4452





Biosystems Design to Enable Next-Generation Biofuels (Summary of Funded Projects) [12/17]

Technologies for Characterizing Molecular and Cellular Systems Relevant to Bioenergy and Environment [9/17]

ASCR BER Exascale Requirements Review [9/17]


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