Multi-Pass Stimulated Raman Scattering Microscopy
Authors:
Shaun C. Burd1* ([email protected]), Joshua Reynolds1, Tzu-Chieh Yen1, Dara Dowlatshahi1,2, Samsuzzoha Mondal1,2, Soichi Wakatsuki1,2, Mark Kasevich1 (PI)
Institutions:
1Stanford University; 2SLAC National Accelerator Laboratory
Abstract
These researchers present results from two multi-pass microscopes that provide metrological advantages in stimulated Raman scattering (SRS) microscopy.
SRS is a nonlinear optical process that is quantitative, bond-specific, and label-free. However, for fragile specimens such as live cells, the required optical intensities result in specimen damage or death, and photon shot noise limits the absolute achievable sensitivity for these dynamic and dose-sensitive samples. A multi-pass microscope interrogates a sample sequentially with the same probe field in a programmable and deterministic fashion, increasing the sensitivity of measurements of weak scatterers ( Juffmann et al. 2016; Israel et al. 2023). In such cases, multi-pass measurements are competitive with or can outperform measurements using squeezed or other quantum states using purely classical resources (Giovannetti et al. 2006).
This research demonstrates multi-passed SRS microscopy using both infrared and visible light modalities. The team quantified the metrological advantage in comparison with a conventional measurement scheme. These quantum-optimal imaging protocols will advance microscopy and flow cytometry for studying the life cycles and interactions of soil microbes and plants and can be shared with the BER science community.
Funding Information
Giovannetti, V., et al. 2006. “Quantum Metrology,” Physical Review Letters 96(1), 010401. DOI:10.1103/ PhysRevLett.96.010401.
Israel, Y., et al. 2023. “Continuous Wave Multi-Pass Imaging Flow Cytometry,” Optica 10(4), 491–6. DOI:10.1364/ OPTICA.482316.
Juffmann, T., et al. 2016. “Multi-Pass Microscopy,” Nature Communications 7, 12858. DOI:10.1038/ncomms12858.