Quantum Optical Microscopy of Biomolecules near Interfaces and Surfaces (QuOMBIS)

Authors:

Mikael Backlund* ([email protected], PI), Simeon Bogdanov, Paul Kwiat, Virginia Lorenz

Institutions:

University of Illinois Urbana-Champaign

Goals

Group members are working to develop three complementary microscopy techniques that exploit quantum correlations in light: Hong-OuMandel interferometric tomography, g⁽²⁾correlation function imaging, and passive and active transverse mode sorting. Researchers will subsequently incorporate these methods into a single platform for tracking and imaging individual and few fluorescently labeled biomolecules, including cellulases, in the context of nearby biological interfaces and surfaces in order to unravel the fundamental processes involved in the conversion of lignocellulosic biomass into renewable fuels.

Abstract

Since the publication of Hooke’s Micrographia in 1665, the scientific disciplines of light microscopy and (sub) cellular biology have progressed in lockstep with one another. Advances in the spatial and temporal resolution, specificity, and sensitivity of optical methods have continually led to new capabilities and insights in biological imaging. The pace of this evolution has quickened in the past century, as a mastery of the physics of light according to Maxwell’s equations has been wielded to more fully exploit classical effects like interference and diffraction. As the classical limits of light microscopy near saturation, however, sustained improvement in bioimaging technology is ultimately untenable without a more fundamental shift in research direction. Just as the field of quantum computing has gained prominence in anticipation of the inevitable breakdown of Moore’s Law, quantum-enabled light microscopy will likely provide the path forward for (sub)cellular biological imaging.

This team aims to help lead this effort by developing three complementary quantum microscopy modalities that each address a different challenge inherent to (sub) cellular microscopy:

Hong-Ou-Mandel Interference Microscopy to enable loss- and noise-tolerant depth imaging with exquisite resolution;
g ⁽²⁾ Microscopy to facilitate orders-of-magnitude sensitivity improvement in focusing and tracking single quantum emitters atop oppressive classical backgrounds at reduced excitation powers; and
Transverse Mode Sorting Microscopy to enable super-resolution microscopy at low excitation powers and high temporal resolution.

This poster presented results demonstrating progress in developing these constituent techniques. The team will ultimately incorporate them into a common imaging platform that can provide access to the many scales of interest in energy-relevant plant and microbial biology. The combined technique, QuOMBIS (Quantum Optical Microscopy of Biomolecules near Interfaces and Surfaces), will be especially powerful for tracking and imaging individual and few fluorescently labeled biomolecules in the context of nearby biological interfaces and surfaces. Upon development of the methods, the team will apply the platform to unravel and harness the enzymatic conversion of biomass into renewable fuels.

Funding Information

This research was supported by the DOE Office of Science, BER program grant DE-SC0023167.